The newborn Trichinella spiralis larvae is capable of penetrating and infecting striated and skeletal muscle fibers of most mammalian species. The parasites often can not be detected by direct microscopical examination.
Serodiagnosis may helpful in such diagnostic difficulties. However, any serological method for the diagnosis of trichinellosis has not yet been available on account of its cross reactivity. The present study was performed to observe the
specifically
reacting antigenic bands of gel filtration antigen of the crude extract Trichinella larval worm and their reaction to the serum IgG antibody obtained from experimentally immunized and infected rats. The localization of specific and cross reacting
antigenic bands were also observed by immunogold labeling method.
@ES The results obtained were as follows:
@EN 1. Serum leveles of IgG antibody began to increase from 6 day after infection.
2. SDS-PAGE profile showed that each antigen consisted of more than 20 polypeptides of molecular mas s450220 kDa in F1, 45-180 kDa in F2, 29-200 kDa in F3, 13-120 kDa in F4, 14-80 kDa in F5, 14-55 kDa in F1, 45-180 kDa in F2. 29-200 kDa in F3,
13-120
kDa in F4, 14-80 kDa in F5, 14-55 kDa in F6, and below 20 kDa in F7
3. Major antigenic bands which reacted to specific IgG antibody infected with Trichinella larvae were 16, 45, 55 and 60 kDa band. A 45 kDa band was identified as common antigen band.
4. Gold particles were labeled on the cuticle, hypodermis surface, esophagus-occupying substance, midgut-occupying substance and α-granule in stichocyte.
5. In the tissue reacted to serum of rabbit immunized with 45 kDa protein as common band, gold particles were labled on the cuticle surface and α, β-granules in stichoyte. Electron micrographs of tissue reacted to rabbit serum immunized with
60
kDa
protein, which was considered as specific antigen band, showed that gold particles were labeled on the hypodermis, esophagus-and midgut-occupying substance and α-granules in stichocyte.
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